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KMID : 0360220110520080970
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Journal of the Korean Ophthalmological Society
2011 Volume.52 No. 8 p.970 ~ p.974
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Effect of Genistein on the Survival and Production of Nitric Oxide in Trabecular Meshwork Cells
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Hong Jung-Heum
Kim Yun-Young
Kim Jae-Woo
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Abstract
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Purpose:To investigate the effects of genistein on the survival and production of nitric oxide in cultured human trabecular meshwork cells (HTMC).
Methods:Primarily cultured HTMC were exposed to 0, 1, and 10 μm genistein using serum-deprived media. Production of nitric oxide and eNOS activity were assessed with the Griess assay and RT-PCR after exposure to genistein for 10 min and one day. Cellular survival was assessed via MTT assay after exposure to genistein for one day.
Results:Genistein significantly increased the production of nitric oxide after exposure for 1 min at 10 μm and for 1 day at 1 μm under serum-deprived conditions. Genistein increased eNOS activity and cellular survival in HTMC.
Conclusions :Genistein increases cellular survival under serum-deprived conditions, accompanied with an increase in nitric oxide production after both short-term and long-term exposures.
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KEYWORD
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Genistein, Nitric oxide, Survival, Trabecular meshwork cells
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